An enzyme-linked immunosorbent assay (ELISA) for measurement of heterophile antibody.

نویسندگان

  • J. F. Hsu
  • A. S. Evans
  • J. C. Niederman
  • L. C. Cenabre
چکیده

An enzyme-linked immunosorbent assay (ELISA) test has been developed for measurement of heterophile antibody. The microtiter test utilizes a bovine erythrocyte monolayer as antigen and anti-human IgM antiserum conjugated with horseradish peroxidase to measure the degree of binding of the heterophile antibody in the test serum with the erythrocytes. A single serum dilution yields quantitative results when read in a spectrophotometer. The ELISA test showed a sensitivity comparable with the immune adherence hemagglutination assay (IAHA) and other heterophile tests, good reproducibility, and high specificity.

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عنوان ژورنال:
  • The Yale Journal of Biology and Medicine

دوره 55  شماره 

صفحات  -

تاریخ انتشار 1982